Homocysteinethiolactone and Paraoxonase Novel markers of diabetic retinopathy
نویسندگان
چکیده
RESEARCH DESIGN AND METHODS — Undiluted vitreous from patients with proliferative diabetic retinopathy (PDR) (n 13) and macular hole (MH) (n 8) was used to determine PON-HCTLase and PON-AREase activity spectrophotometrically. HCTL levels were detected by liquid chromatography–tandem mass spectrometry. In vitro studies were done in primary cultures of bovine retinal capillary endothelial cells (BRECs) to determine the doseand time-dependent effect of HCTL and homocysteine (Hcys) on PON-HCTLase activity, as well as to determine mRNA expression of PON by RT-PCR.
منابع مشابه
Homocysteinethiolactone and Paraoxonase
OBJECTIVE Paraoxonase (PON) exhibits esterase activity (PON-AREase) and lactonase activity (PON-HCTLase), which prevent LDL oxidation and detoxify homocysteine thiolactone (HCTL). The role of HCTL and PON-HCTLase as a risk factor for the microvascular complication in diabetic retinopathy at the level of vitreous has not been investigated. RESEARCH DESIGN AND METHODS Undiluted vitreous from pa...
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